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The most practical option for sensing near the single-molecule level for a variety of analytes in solution or air, lending to an array of applications ranging from trace gas detection to automated protein identification.

Even simple (diatomic) molecules can have complex and reproducible vibrational fingerprints.

DeltaNu Inspector Raman Diesel Fuel Spectrum.

Instrumentation $10,000 - $180,000 Image source: (New York Talk Exchange) Numbers:.

Consumables $50 to $750 per analysis $1 million market annually.

In-vivo glucose sensing Faraday Discuss., 132, 9 (2006)

Single molecule spectroscopy is possible S.M.

Near-IR excitation and biocompatability allow.

Plasmon resonances allow for sensor tunability.

Unique vibrational spectra distinguishes molecules.

Depends on local geometry of adsorption site K.

Adsorbed molecules see increased field.

Plasmon resonance leads to local field enhancement near the surface.

Mater., 13 1501 (2001)ДЎ0-250 nm SERS Enhancement Enhancing SERS substrates

Coupled to surface plasmon excitation of metal nanostructures Tunable resonances: Shape- and Size-effects A.J.

Based on metal-molecule charge-transfer effects.

Rev., 99 2957 (1999)ДЄway from plasmon resonance At plasmon resonance SERS Enhancement Moskovits,, Reviews of Modern Physics, 57 3 (1985) K. about structural data! Gelder, et al., J.

Compared to Rayleigh scattering / fluorescence Provides rich info.

Based on symmetry elements of polarizability tensor.

Raman Adapted from ДЄdenine cytosine guanine thymine uracil Raman Spectroscopy The SERS Solution Adsorption Excitation Detection

Immunological Methods Vol 292, Issues 1-2, September 2004, pp.35-42 Reflectometric interference spectroscopy (RIFS) Protein concentration determined spectrophotometrically and active antibody concentration determined by biosensor and ELISA for 9 sequentially eluted fractions. Shift in characteristic interference spectrum is transformed into a signal curve.

The reflected beams superimpose and change optical thickness of the transducer by binding events onto the surface.

Reflectometric interference spectroscopy (RIFS)

Direct monitoring of analyte in solution Incorporation of spectroscopically responsive monomers into the matrix Competition assays using labeled ligands Polymer International, Vol 56( (4), pp.

Molecularly Imprinted Polymers as Optical Sensors Schematic representation of molecular imprinting Distribution of binding affinities in MIP vs.

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Total internal reflectance fluorescence (TIRF) biosensor TIR Evanescent wave Д®pifluorescence TIRF Typical TIRF Sensogram Advantages High Signal to noise ratio (very little secondary emission from bulk solution) Highly robust, low cost, portable Drawbacks Need for labels High cross-reactivity (hence not easy to multiplex) Vast Technology Landscape Diverse Applications Financial and market considerations of SERS.Surfaced-enhanced Raman scattering (SERS).Technology Landscape: Optical techniques for biosensing.SERS-based Biosensors James Krier, Lalitha Muthusubramaniam Kevin Wang, Douglas Detert Final Presentation EE235: Nanofabrication May 12, 2009